AUTHORS
Marie TAUTOU, Charline PERROUIN, Jade RUARD, Léna MARLHOUX, Mélina GAUTHIER, Pierre-Antoine CHOFFOUR, Doriane MATHE, Charles DUMONTET
INTRODUCTION
This new luminescence-based method allows us to track tumors and metastases in in vivo models using our Ivis Imager®.
MATERIALS & METHODS
For the development of the luciferase bioluminescent cell lines tumor monitoring, we used SCID CB17 and NSG mice, with the MDA-MB-231 cell line, a model of breast cancer, and DEL cell line, a model of lymphoma.
Compared to the fluorescence method, luminescence is a more sensitive approach allowing whole body imaging and the detection of deep tumors and metastasis in live mice (1).
RESULTS
Monitoring of a breast cancer line – (MDA-MB-231 Luc+)
• Correlation between tumor volume measured with a caliper and the luciferase bioluminescent signal
• Consistent tumor growth after 2 months of monitoring
Monitoring of a metastasis from a T Lymphoma – (DEL Luc+)
• Detection of a second signal different from the primary tumor, localized under the left paw
• Collection of the lymph node and dissociation of the cells for FACS analysis
• Validation of the second signal resulting of a metastasis from primary tumor
CONCLUSION
We have developed luciferase bioluminescent (Luc+) cell lines by transduction, and we have conducted a first longitudinal follow-up of the MDA-MB-231 cell line (breast cancer), and a second monitoring of the DEL cell line (lymphoma cancer), injected subcutaneously, and the associated metastasis.
The images obtained with the Ivis Imager® allow us to regularly monitor tumors and potential metastases over time and without loss of luminescence.
We also offer alternative experimental designs with the Ivis Imager®:
• Tracking of a luminescent compound to follow its biodistribution
• Orthotopic injection for a more precise and non-invasive follow-up with the classic method (caliper)
• Development of on-demand models
REFERENCES
1. Genevois, Coralie, et al. « In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography ». International Journal of Molecular Sciences, vol. 17, no 11, octobre 2016, p. 1815.
2. Liu, Shirley, et al. « Brightening up Biology: Advances in Luciferase Systems for in Vivo Imaging ». ACS Chemical Biology, vol. 16, no 12, décembre 2021, p. 2707‑18.
