Syngeneic breast cancer tumour model – 4T1

4T1 cells

Mouse 4T1 cells were isolated from the spontaneous tumour in the mammary gland of a BALB/C mouse with the animal equivalent of a stage IV breast cancer.

Tumour growth in vivo

The cells were collected from a tissue culture flask and injected subcutaneously in the right flank of BALB/C mice. The resulting tumours were monitored by measuring two diameters with calipers, and extrapolating the volume to a sphere.

The mice bearing 4T1 tumours can be treated by intra-peritoneal, intra-venous, intra-tumoral or subcutaneous injection of the compounds. Per os administration is also possible.

Figure 1: (view pdf)

Tumour growth curve of the 4T1 cells as subcutaneous tumours, Mean ± SEM

Antineo can also implant this model orthotopically.

Drug Response – Doxorubicin

Figure 2: (view pdf)
Effect of Doxorubicin at 1mg/kg or 2mg/kg on 4T1 tumour growth, Mean ± SEM

Drug Response – Anti-PD1

Figure 3: (view pdf)
Partial response of Anti-PD1 at 12.5mg/kg on 4T1 tumour growth, Mean ± SEM

Drug Response – Anti-PDL1

Anti-PDL1 12.5 mg/kg no response and toxic.

Immunophenotyping data of the lymphoid and myeloid lineage are available upon request.

Mammary Gland Syngeneic breast cancer tumour model – 4T1

Mammary Gland