Antineo : Mammary Gland Syngeneic tumour model – 4T1
▪ 4T1 cells
Mouse 4T1 cells were isolated from the spontaneous tumour in the mammary gland of a BALB/C mouse with the animal equivalent of a stage IV breast cancer.
▪ Tumour growth in vivo
The cells were collected from a tissue culture flask and injected subcutaneously in the right flank of BALB/C mice. The resulting tumours were monitored by measuring two diameters with calipers, and extrapolating the volume to a sphere.
The mice bearing 4T1 tumours can be treated by intra-peritoneal, intra-venous, intra-tumoral or subcutaneous injection of the compounds. Per os administration is also possible.
Figure 1: (View PDF)
Tumour growth curve of the 4T1 cells as subcutaneous tumours
Mean ± SEM (n=6; take rate 100%)
Antineo can also implant this model orthotopically.
▪ Drug Reponses
Anti-PD1 12.5 mg/kg → Partial Response
Anti-PDL1 12.5 mg/kg → no response and toxic
Immunophenotyping data of the lymphoid and myeloid lineage are available upon request.