Antineo : Diffuse Large B Cells Lymphoma tumour model – Toledo

  • Toledo cells

Human Toledo cells were isolated from the peripheral blood of a patient with non-hogkin’s DLBC lymphoma.

  • Tumour growth in vivo

The cells were collected from a tissue culture flask and injected subcutaneously in the right flank of SCID-CB17 mice. The resulting tumours were monitored by measuring two diameters with calipers, and extrapolating the volume to a sphere.  The mice bearing Toledo tumours can be treated by intra-peritoneal, intra-venous, intra-tumoral  or subcutaneous injection of the compounds. Per os administration is also possible.

Figure 1: (View PDF)
Tumour growth curve of the Toledo cells as xenograft Mean ± SEM (n=3; take rate 100%)

Antineo can also perform an intra-medullar implantation.

  • Standard-Of-Care Drug Reponses

rituximab 30 mg/kg qw → Response

Figure 2: (View PDF)

Effect of rituximab treatment on Toledo tumour growth Mean ± SEM (n=3 per group; take rate 100%)

A Toledo resistant to rituximab model, developed in vivo without genetic modifications, are available at Antineo (model ID Toledo rituxR).

 

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